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:: Volume 3, Issue 4 (Winter 2014) ::
J Fasa Univ Med Sci 2014, 3(4): 380-386 Back to browse issues page
Monitoring the Various Types of Clostridium botulinumin in Four Kinds of Food Stuffs Using Multiplex PCR
Mohammad Vahid Sadeghi Sarvestani1, Saeid Hosseinzadeh 2, Maryam Poormontaseri1, Mehdi Fazeli1
1- Shiraz University
2- Shiraz University , hosseinzadeh@shirazu.ac.ir
Abstract:   (10165 Views)
Background &Objective: Food poisoning (FP) caused by C. botulinum is the most serious feature of FP inpeople consuming the contaminated foodstuffs (Canned meat, vegetarian foods, dairy products and seafood products). Botulism is basically detected by the identification of live bacteria and/or its toxins. Among various types of microorganisms (i.e. A, B, C1, C2, D, E, F), serotypes A, B, E and F are considered as the main human pathogens. The present study was aimed at investigating the possible roles of various foodstuffs to induce the food intoxication and also to compare the culture and molecular assays for identifying the microorganism.
Materials &Methods: Three Lab techniques including biochemical, culture (enriched in TPGY and cooked meat medium) and MPCR were used to detect C. botulinum in the samples. As the molecular based techniques have recently employed for the rapid and reliable identification of the bacteria and its toxins, the PCR assay, using three pairs of primers were designed and optimized to identify A, B and E strains in the contaminated specimens. The PCR was able to amplify 782, 205 and 389 bp genes specified for A, B and E types of the bacteria, respectively.
Results: Total number of 290 specimens including fish, honey,"kashk"and"Dough" were tested, in which 5%, 4%, 2.5% and 1.25%, were found positive, respectively. Using selective culture of the specimens on the enriched samples, it was shown that just four samples were found positive.
Conclusion: As a final conclusion, the molecular based techniques are recommended as a reliable tool to detect C. botulinum and, its toxins and spores in foodstuffs. Moreover, it is strongly advised to use it in food microbial Lab and also the epidemiological surveys.
Keywords: Clostridium botulinum, Multiplex PCR, Food stuffs
Full-Text [PDF 673 kb]   (5972 Downloads)    
Type of Study: Research | Subject: Microbiology (Food Industry)
Received: 2013/05/6 | Accepted: 2013/09/21 | Published: 2014/03/16
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Sadeghi Sarvestani M V, Hosseinzadeh S, Poormontaseri M, Fazeli M. Monitoring the Various Types of Clostridium botulinumin in Four Kinds of Food Stuffs Using Multiplex PCR. J Fasa Univ Med Sci. 2014; 3 (4) :380-386
URL: http://journal.fums.ac.ir/article-1-187-en.html


Volume 3, Issue 4 (Winter 2014) Back to browse issues page
Journal of Fasa University of Medical Sciences
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