Evaluation of Serum Specific Antibody against Recombinant ESAT-6 Antigen in Patients with Tuberculosis and Comparing to Normal Controls
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Homeira Izadi1, Nooshin Sohrabi *2, Majid Tebianian3, Nader Mosavari3, Mehdi Mahdavi4 |
1- Department of Biology, Payame Noor University, Tehran, Iran 2- Department of Biology, Payame Noor University, Tehran, Iran , nsohrabi75@yahoo.com 3- Razi Vaccine and Serum Research institute, Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran 4- Department of Immunology, Pasteur Institute of Iran, Tehran, Iran |
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Abstract: (7369 Views) |
Background & Objective: Tuberculosis (TB) is a zoonotic disease which is caused by Mycobacterium tuberculosis. Because of common structural and secretory antigens between pathogen and nonpathogenic mycobacterium, the specific diagnosis of TB is difficult. Therefore, it is very important to find a new method with high specificity and sensitivity for accurate and rapid diagnosis of tuberculosis. In this study, the serodiagnostic potential of Mycobacterium tuberculosis recombinant ESAT-6 in TB infected patients was evaluated by Enzyme Linked Immunosorbent Assay (ELISA).
Materials & Methods: 55 TB patients with active disease and 28 healthy controls have been collected and evaluated in different dilutions in ELISA methods for the presence of specific anti-ESAT-6 antibody. The specificity and the sensitivity of this method was compared with the culture test.
Results: TB patients have high levels of specific antibody against ESAT-6 antigens. The specificity and the sensitivity of this method was calculated as 80.90% and 85.45%, respectively.
Conclusion: These findings provide useful information on the importance of ESAT-6 protein and suggested this serologic test as a good alternative method for rapid and prefect diagnosis of tuberculosis. |
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Keywords: Mycobacterium tuberculosis, ESAT-6, Antibody, ELISA |
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Full-Text [PDF 1276 kb]
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Type of Study: Research |
Subject:
Microbiology Received: 2016/06/18 | Accepted: 2016/11/1 | Published: 2017/03/8
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