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:: Volume 2, Issue 1 (Spiring 2012) ::
J Fasa Univ Med Sci 2012, 2(1): 19-25 Back to browse issues page
Molecular Typing of Salmonella paratyphi B and Salmonella paratyphi C Isolates from Clinical Samples in Iran
Fahimeh Baghbani-arani 1, Mercedeh Tajbakhsh, Atiyeh Hashemi Soltaniyeh, Bahareh Rajaei, Seyed Davar Siadat, Mohamahreza Aghasadeghi, Seyed Mehdi Sadat
1- , fbaghbani@iauvaramin.ac.ir
Abstract:   (17040 Views)

Background & Objective: Molecular typing is an important tool in surveillance and outbreak investigations of human Salmonella infections. In this study, Subtyping of Salmonella Paratyphi B and C isolates derived from Iranian patients was carried out by RAPD-PCR to assess the extent of genetic diversity of these isolates.

Materials & Methods: Fourteen Salmonella isolates including 6 strains of Salmonella paratyphi B and 8 strains of Salmonella paratyphi C were characterized using RAPD-PCR. Two arbitrary primers, namely OPP-16 and P1254 were used for RAPD analysis and the dendrograms were constructed with NTsys 2.0 computer software.

Results: Both primers showed high discriminatory power in differentiating of the related strains of Salmonella. The dendrograms constructed based on RAPD-PCR profiles (with both primers) involving 14 salmonella strains revealed 4 distinct patterns, indicating that these isolates are genetically heterogeneous. Furthermore, a good correlation was not observed between the serotype and the molecular profiles obtained from RAPD data of the Salmonella isolates.

Conclusion: The findings of the present study verify the usefulness of RAPD-PCR in characterizing and comparing strains of Salmonella Paratyphi B and C.

Keywords: Salmonella, RAPD-PCR, NTsys, Genetic Diversity
Full-Text [PDF 821 kb]   (2949 Downloads)    
Type of Study: Research | Subject: Medical Genetics
Received: 2013/02/11 | Accepted: 2013/09/14 | Published: 2013/09/14
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Baghbani-arani F, Tajbakhsh M, Hashemi Soltaniyeh A, Rajaei B, Siadat S D, Aghasadeghi M et al . Molecular Typing of Salmonella paratyphi B and Salmonella paratyphi C Isolates from Clinical Samples in Iran. J Fasa Univ Med Sci. 2012; 2 (1) :19-25
URL: http://jabs.fums.ac.ir/article-1-72-en.html


Volume 2, Issue 1 (Spiring 2012) Back to browse issues page
Journal of Fasa University of Medical Sciences
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