Saman Yousefi Saggezi, Akbar Azamian Jazi, Roohullah Hemmati, Nahid Jivad,
Volume 9, Issue 2 (7-2019)
Abstract
Background & Objective: Several evidence has indicated the effective role of exercise on Multiple sclerosis (MS) patients. An aberrant expression of some Micro-RNAs such as increasing miR-301a in MS, with an impact on inflammatory pathways leading to the creation of inflammation in these patients. The purpose of this study was to investigate the effect of a combined eight-week (aerobic and resistance) exercise on expression of miR -301a and some indicators of motor function in women with multiple sclerosis.
Materials & Methods: A total of 22 women with MS, who were randomly divided into experimental and control groups, participated in this study. The experimental group performed 8 weeks (4 days per week) of combined training. Expression of miR -301a was measured before and after the training period using Real-Time PCR method. Pre- and post-intervention scores of tests (TUG), (6MWT), (10–MWT) and status scale (EDSS) were independent t-test recorded and analyzed.
Results: The results of this study showed, the expression of miR -301a was significantly different between the two groups (P = 0.001). Tests TUG (P = 0.001), 6MWT (P = 0.002) and degree of disability EDSS (P = 0.015), were significantly different between the two groups. There was no significant difference between the 10MWT test between the two group (P = 0.678).
Conclusion: The combined exercise for women with MS have been beneficial and improve motor function and reduce the expression of miR-301a. So, do it, plays an important role in reducing inflammation and prevent the progression of the disease Ms.
Hamid ِِِdaneshmandi, Akbar Azamian Jazi, Behnam Ghasemi,
Volume 10, Issue 4 (11-2020)
Abstract
Background & Objective: Patients with diabetes have impaired beta cells function and insulin secretion. Exercise training may have a significant role in the improvement of these disorders through the expression of the specific genes. Therefore, the present study aimed to investigate the effects of eight weeks of moderate-intensity continuous and resistance training on the glycogen synthase kinase-3 beta (GSK-3β) gene expression, and serum glucose and insulin levels in streptozotocin-induced diabetic rats
Materials & Methods: 24 rats were divided into four groups: healthy control, diabetic control, diabetic + moderate-intensity continuous training, and diabetic + moderate-intensity resistance training. Diabetes was induced by intravenous injection of 110 mg nicotinamide, and 40 mg streptozotocin per kg of body weight. Exercise training intervention was performed for eight weeks. Expression of GSK-3β gene in pancreatic tissue was measured by real-time PCR, and serum glucose and insulin levels were measured by ELISA. Data were analyzed using one-way analysis of variance.
Results: Insulin levels in the resistance and continuous training groups increased compared to the diabetic control group (p<0.05) and decreased compared to the healthy control group (p<0.05). Also, blood glucose levels in the resistance and continuous training groups decreased compared to the diabetic control group (p<0.05) and increased compared to the healthy control group (p<0.05). Moreover, the results showed a significant decrease in GSK-3β gene expression in the resistance and continuous training groups compared to the healthy control and diabetic control groups (p<0.05).
Conclusion: It seems that insulin secretion can increase through the decrease in GSK-3β expression following the eight weeks of moderate-intensity continuous and resistance training.
