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:: Volume 4, Issue 3 (Autumn 2014) ::
J Fasa Univ Med Sci 2014, 4(3): 327-334 Back to browse issues page
Effects of Several Common Antifungal Drugs (Clotrimazole, Miconazole, Fluconazole) alone and in Combination with Amphotericin B on Candida Species Isolated from Chronic Candidal Vulvovaginitis
Efate alsadat Shojaei1, Mehraban Falahati 2, Faride Zaini1, Parivash Kord bache1, Parvaneh Rahimi moghadam3, Mohammad reza Aghamirian4, Shima Nozari5, Ayesheh Makhdoomi4, Sekhavatv Ameri1, Sanam Afshar moghadam1
1- Department of Parasitology and Mycology, Iran University of Medical Sciences, Tehran, Iran.
2- Department of Parasitology and Mycology, Iran University of Medical Sciences, Tehran, Iran. , mehrabanfalahati@yahoo.com
3- Department of Pharmacology, Iran University of Medical Sciences, Tehran, Iran.
4- Department of Parasitology and Mycology, Qazvin University of Medical Sciences.
5- Department of Parasitology and Mycology, Bandar Abbas University of Medical sciences
Abstract:   (10475 Views)

 Background& Objective: Candidal vulvovaginitis occurs in female genital by the over growth of candida especially candida albicans. This infection may be resistant to therapy and occasionally becomes chronic. In some patients, this form of infection is recurrent. Moreover, discovering new antifungal drugs and the use of these drugs in new methods encourage mycologists to use these techniques. Therefore, according to the above methods, this research investigated effects of several common antifungal drugs with the use of combination methods against candida species isolated from chronic candidal vulvovaginitis.

Materials& Methods: This study carried out on 19 strains of candida albicans that were isolated from recurrent vulvovaginitis. The effects of clotrimazole, miconazole, fluconazole were assessed separately and in combination with amphotericin B on candida albicans using micro dilution methods.

Results: The mean Minimum Inhibitory Concentration (MIC) of clotrimazole, miconazole, fluconazole, and amphotericin B were 7.05, 10.7,47, and 0.6 µgr/ml, respectively after 48 hours incubating. Then, the azole antifungal drugs were evaluated in combination with amphotericin B. The two combinations of fluconazole + amphotericin and miconazole + amphotericin with the Fungicidal Inhibitory Concentrations (FIC) of 0.6 and 1, respectively, attracted the most attentions. In addition, comparing the antifungal effects, the most effective drug was amphotericin B with the Minimum Fungicidal Concentration (MFC) of 1.18.

Conclusion: In this study, amphotericin B with the least MIC had the best antifungal effect. The most and the least synergic effects of combination therapy were for fluconazole + amphotericin B and Miconazole + amphotericin B, respectively.

Keywords: Chronic candidal vulvovaginitis, Drugs combination, Synergism, Micro dilution broth, Clotrimazole, Miconazole, Fluconazole, Amphotericin B
Full-Text [PDF 1185 kb]   (4840 Downloads)    
Type of Study: Research | Subject: Mycology
Received: 2013/09/20 | Accepted: 2014/07/29 | Published: 2014/12/20
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shojaei E A, Falahati M, zaini F, kord bache P, rahimi moghadam P, aghamirian M R, et al . Effects of Several Common Antifungal Drugs (Clotrimazole, Miconazole, Fluconazole) alone and in Combination with Amphotericin B on Candida Species Isolated from Chronic Candidal Vulvovaginitis. J Fasa Univ Med Sci. 2014; 4 (3) :327-334
URL: http://journal.fums.ac.ir/article-1-108-en.html


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