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:: Volume 9, Issue 2 (summer 2019) ::
J Fasa Univ Med Sci 2019, 9(2): 1443-1456 Back to browse issues page
Preparation of Hydrocortisone Conjugated Polyethylenimine Nanocarriers and its Characterizations for Potential Application in Targeted Gene Delivery
Hossein Sadeghpour1 , Elaheh Sabet Dr2 , Ali Dehshahri 3
1- Department of Pharmaceutical Chemistry, Faculty of pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran
2- . Department of Pharmaceutical Chemistry, Faculty of pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran
3- Department of Pharmaceutical Biotechnology, Faculty of pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran , dehshahria@sums.ac.ir
Abstract:   (261 Views)
Background & objective: nowadays, gene therapy has been considered as a novel therapeutic approach. Particularly, following the introduction of immunotherapy and gene editing using CRISPR/Cas9 platform great attention has been directed to gene therapy. However, finding an efficient carrier with low toxicity is still one of the major challenges for researchers. Among non-viral gene carriers, polyethyleneimine (PEI) could be considered as the most studied polycationic compound for gene delivery. However, toxicity, lack of targeting and non-specific interactions with serum components limit its wide application. Therefore, PEI structure must be modified through chemical conjugations. In this study, conjugation of hydrocortisone on the polymer structure was carried out in order to direct the carriers into the cell nucleus.
Materials & Methods: hydrocortisone was conjugated onto polyethylenimine followed by the grafting of the targeted PEI domain onto another unmodified polymer via succinic acid linker. The conjugate w::as char::acterized in terms of buffering capacity, zeta potential, particle size, protection of DNA against enzymes and condensation ability.
Results: The results demonstrated that the conjugates could condense plasmid DNA successfully and form nanoparticles with the size of around 300 nm. Meanwhile, the conjugates showed higher buffering capacity compared with the unmodified polymer. Also, the protection ability of the conjugates was significant rather than the parent polymer.
Conclusion: According to the results, design and synthesis of the PEI derivative with two separated domains responsible for targeting and plasmid condensation could be considered as an efficient strategy to create nano gene delivery systems.
 
 
Keywords: polyethylenimine, hydrocortisone, targeted gene delivery, polymeric nanoparticle
Full-Text [PDF 874 kb]   (38 Downloads)    
Type of Study: Research | Subject: Nanomedicine
Received: 2019/01/1 | Accepted: 2019/05/4 | Published: 2019/09/18
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Sadeghpour H, Sabet E, Dehshahri A. Preparation of Hydrocortisone Conjugated Polyethylenimine Nanocarriers and its Characterizations for Potential Application in Targeted Gene Delivery . J Fasa Univ Med Sci. 2019; 9 (2) :1443-1456
URL: http://journal.fums.ac.ir/article-1-1894-en.html


Volume 9, Issue 2 (summer 2019) Back to browse issues page
Journal of Fasa University of Medical Sciences
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