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:: Volume 10, Issue 2 (Summer 2020) ::
J Fasa Univ Med Sci 2020, 10(2): 2294-2307 Back to browse issues page
The Effects of Silver Nanoparticles Coatings in Effective Drug Delivery: Human Serum Albumin Interaction
Azadeh Hekmat 1, Zahra Roshani2
1- Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran , hekmat@ut.ac.ir
2- Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
Abstract:   (1350 Views)
 Background & Objective: Silver nanoparticles (AgNPs) are increasingly used in nanomedicine and pharmacy due to their valuable properties. AgNPs are synthesized in various ways. The amount of drug binding to Human Serum Albumin (HSA) as the most abundant protein in the circulatory system would affect the drug’s therapeutic concentration and its availability for intracellular targets. Thus, the purpose of this study was to investigate the effect of coating in the synthesis of AgNPs on their ability to interact with HSA.
Materials & Methods: Fluorescence emission spectroscopy, circular dichroism (CD) spectroscopy, and Zeta potential analysis were used to study the structural effects of HSA after interaction with AgNPs with ethylene glycol dispersion matrix. AgNPs contains sodium citrate stabilizer and uncoated AgNPs.
Results: The fluorescence quenching indicated that AgNPs with ethylene glycol dispersion matrix interacted with HSA with a higher binding constant value compared with two other AgNPs. The CD spectra indicated a reduction in α-helix content of HSA especially after the addition of AgNPs with ethylene glycol dispersion matrix (from 51.45% to 41.71%). The zeta potential analysis showed that the surface charge of HSA was reduced especially after the addition of AgNPs with ethylene glycol dispersion matrix (from -16.73 mV to -11.79 mV).
Conclusion: This study showed that AgNPs with ethylene glycol dispersion matrix interact with HSA more strongly compared with two other AgNPs. This study indicated that the type of coating of AgNPs affects the HSA interaction and consequently affects the nanoparticles transportation and distribution in the blood.

 
Keywords: Human Serum Albumin (HSA), Silver nanoparticles, Synthesis​​​​​​​
Full-Text [PDF 1719 kb]   (446 Downloads)    
Type of Study: Research | Subject: Biophysics
Received: 2019/09/12 | Accepted: 2019/12/23 | Published: 2020/09/19
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Hekmat A, Roshani Z. The Effects of Silver Nanoparticles Coatings in Effective Drug Delivery: Human Serum Albumin Interaction. J Fasa Univ Med Sci. 2020; 10 (2) :2294-2307
URL: http://jabs.fums.ac.ir/article-1-2117-en.html


Volume 10, Issue 2 (Summer 2020) Back to browse issues page
Journal of Fasa University of Medical Sciences
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