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:: Volume 10, Issue 4 (Winter 2020) ::
J Fasa Univ Med Sci 2020, 10(4): 2768-2774 Back to browse issues page
 The Effect of Lactobacillus Casei-Free Floating Fraction on the Expression of Class I Integron Gene in Clinical Isolates of Acinetobacter Baumannii by Real Time PCR
Amir Jalilian1 , Gholamali Moradli 2, Kumarss Amini1
1- Department of Microbiology, Faculty of Basic Sciences, Saveh Branch, Islamic Azad University, Saveh, Iran
2- Department of Microbiology, Faculty of Basic Sciences, Saveh Branch, Islamic Azad University, Saveh, Iran , moradli.mic@gmail.com
Abstract:   (348 Views)
Background & Objective: The most common way for the spread of resistance genes and the emergence of multidrug resistance (MDR)-Acinetobacter baumannii species is the presence of genetic elements called integrons. Therefore, in recent years, the use of probiotics has been recommended to reduce the complications of resistant bacteria and treatment failure. Therefore, the aim of this study was to investigate the effect of probiotic Lactobacillus casei supernatant on the expression of class 1 integron gene in A. baumannii isolates by Real time PCR.
Material & methods: Sixty clinical samples of blood, respiratory secretions, urine, and ulcers were collected from hospitals in Saveh, Iran. The presence of the intI gene was evaluated by PCR. After treatment with the sub-MIC strains of L. casei supernatant, mRNA was extracted and Real-time PCR was performed to determine the level of integron gene expression and finally the expression level was determined using Δct method. Expression analysis was performed by relative mRNA expression compared to the standard strain and it was performed in SPSS version 18 using t-test statistical analysis.
Results: Out of 12 isolates of A. baumannii, the prevalence of IntI gene was 100%. The fold change for the intI gene was -1.18, indicating that it decreased in the treated group compared to the untreated group (P <0.05).
Conclusion: The results showed that the probiotic supernatant was able to reduce the expression of the intI gene; therefore, the use of probiotics as a supplement or alternative therapy in the drug-resistance microbial infections is recommended.
 
Keywords: Acinetobacter baumannii, Integron, Lactobacillus casei, Real-time PCR
Full-Text [PDF 1094 kb]   (69 Downloads)    
Type of Study: Research | Subject: Medical Biotechnology
Received: 2020/04/19 | Accepted: 2020/09/29 | Published: 2021/01/29
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Jalilian A, Moradli G, Amini K.  The Effect of Lactobacillus Casei-Free Floating Fraction on the Expression of Class I Integron Gene in Clinical Isolates of Acinetobacter Baumannii by Real Time PCR. J Fasa Univ Med Sci. 2020; 10 (4) :2768-2774
URL: http://jabs.fums.ac.ir/article-1-2299-en.html


Volume 10, Issue 4 (Winter 2020) Back to browse issues page
Journal of Fasa University of Medical Sciences
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