Volume 10, Issue 4 (11-2020)                   JABS 2020, 10(4): 2665-2672 | Back to browse issues page

XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Jafari Shibani F, Hosseini S E, Mehrabani D. Investigating the Effect of Methamphetamine Toxicity on Mesenchymal Stem Cells Derived from Adipose Tissue in Rats. JABS 2020; 10 (4) :2665-2672
URL: http://jabs.fums.ac.ir/article-1-2353-en.html
1- Department of Biology, Shiraz Branch, Islamic Azad University, Shiraz, Iran
2- Department of Biology, Shiraz Branch, Islamic Azad University, Shiraz, Iran , ebrahim.hossini@yahoo.com
3- Stem Cell Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran,
Abstract:   (1959 Views)
Background & Objective: Methamphetamine is a hallucinogenic and addictive substance that is widely used worldwide. Cellular toxicity in various cells, especially in nerve cells, is one of the reported effects of this psychoactive substance. Therefore, this study aimed to investigate the effect of methamphetamine cytotoxicity on adult mesenchymal stem cells
Materials & Methods: In this experimental study, mesenchymal stem cells were extracted from the adipose tissue of adult male rats of the Wistar breed and after culturing, their being mesenchymal was confirmed by flowcytometry method using CD34-RPE, CD90-RPE and CD105-RPE conjugate antibodies. In the third passage of cell culture, the effect of methamphetamine toxicity at a concentration of 0.6 mmol during 1 to 7 days on the growth process of these cells was investigated by MTT test.
Results: The cells detached from the adipose tissue were completely adhered to the floor of the flask 24 hours after being transferred to the cell culture flask. The results of flowcytometry showed that the expression of negative endothelial surface marker (CD34) and the expression of mesenchymal cell markers (CD90, CD105) were positive, which confirmed the basic identity of the extracted cells. The results of the MTT test also showed a significant reduction in the growth of cells treated with 0.6 mmol methamphetamine compared with the control group at p <0.05.
Conclusion: Cells isolated from adipose tissue are the basic mesenchymal type in which methamphetamine can induce the effects of cell toxicity and inhibit growth.
 
Full-Text [PDF 1110 kb]   (684 Downloads)    
Type of Study: Research | Subject: Physiology
Received: 2020/06/4 | Accepted: 2020/08/2 | Published: 2021/01/29

Send email to the article author


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2024 CC BY-NC 4.0 | Journal of Advanced Biomedical Sciences

Designed & Developed by: Yektaweb

Creative Commons License
This work is licensed under a Creative Commons — Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)