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:: Volume 12, Issue 2 (Spring(2022) 2022) ::
JABS 2022, 12(2): 194-202 Back to browse issues page
The Protective Effect of Curcumin on the Proliferation and Colonization of Spermatogonial ‎Stem Cells ‎in Gamma-Irradiated Rats
Sareh Azarmi1 , ‎khosro Hoseini ‎pajooh 2, Parviz Tajik3 , ‎Marziyeh Lashkari4
1- Faculty of Veterinary Medicine, Tehran University, Tehran, Iran
2- Department of Biotechnology, Iranian Research Organization for Science and Technology (IROST), Tehran, Iran , pajooh@irost.ir
3- Department of Theriogenology, Faculty of Veterinary Medicine, Tehran University, Tehran, Iran
4- Department of Radiotherapy, Tehran University of Medical Sciences, Tehran, Iran
Abstract:   (64 Views)
Background & Objective: One of the side effects of radiotherapy can be damage to spermatogonial stem cells that ‎may lead to spermatogenesis disorders and sterility. Protective effects of curcumin on normal cells ‎against radiotherapy side effects have already been shown. In the current study, the protective effects of curcumin ‎on the spermatogonial stem cells against gamma radiation were ‎evaluated.‎
Materials & Methods: This study was done on 50 adult rats in 10 experimental groups. Four groups were ‎injected 0, 25, 50, or 100mg/kg of curcumin in 1ml olive oil for 15 days intraperitoneally, then exposed to radiation ‎at 2 Gy on the next day. Also, four groups were treated like above but without radiation; and two groups as control with and without radiation.  The day after radiation, all of the rats were euthanatized, their testes were removed, and they underwent enzymatic digestion to co-culture spermatogonial stem cells. After 12 days, the colonization of spermatogonial stem cells was assessed. ‎
Results: There was a significant decrease in the colonization of spermatogonial stem cell proliferation ‎in groups that had taken radiation but not curcumin. There was a significant increase ‎ in the colonization of spermatogonial stem cells in the group which had taken radiation whit maximum curcumin compared with the other irradiated groups and ‎was similar to non-irradiated control animals. Colonization of spermatogonial stem cells in non-irradiated animals treated with curcumin had increased compared with control groups.‎
Conclusion: Injection of curcumin can protect spermatogonial stem cells against ‎radiation. Thus, curcumin may prevent sterility in men who undergo radiotherapy. ‎
 
Keywords: Curcumin, Radiation therapy, Radiation protective agents, Spermatogonial stem cells, Sterility
Full-Text [PDF 1008 kb]   (14 Downloads)    
Type of Study: Research | Subject: Pharmaceutical Plants
Received: 2021/04/17 | Accepted: 2022/03/6 | Published: 2022/06/28
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Azarmi S, Hoseini ‎pajooh ‎, Tajik P, Lashkari ‎. The Protective Effect of Curcumin on the Proliferation and Colonization of Spermatogonial ‎Stem Cells ‎in Gamma-Irradiated Rats. JABS. 2022; 12 (2) :194-202
URL: http://jabs.fums.ac.ir/article-1-2659-en.html


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Volume 12, Issue 2 (Spring(2022) 2022) Back to browse issues page
Journal of Fasa University of Medical Sciences
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