Volume 13, Issue 4 (9-2023)                   JABS 2023, 13(4): 252-264 | Back to browse issues page

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Ghasemian A, Nournia E, Nikfar G, Kohansal M, Ghorbani M, Salahi A et al . Advantages and Challenges of CRISPR-Cas9 Applications in Animal Modeling: A Concise Review. JABS 2023; 13 (4) :252-264
URL: http://jabs.fums.ac.ir/article-1-2947-en.html
Advantages and Challenges of CRISPR-Cas9 Applications in Animal Modeling: A Concise Review
Abdolmajid Ghasemian * 1, Ensieh Nournia2 , Ghasem Nikfar3 , Maryam Kohansal4 , Mohammad Ghorbani5 , Afsaneh Salahi4 , Mojtaba Memariani6
1- Noncommunicable Diseases Research Center, Fasa University of Medical Sciences, Fasa, Iran , majidghasemian86@gmail.com
2- Cardiologist at Be'sat Hospital, Hamedan University of Medical Sciences, Hamedan, Iran
3- Clinical Research Development Unit, Valiasr Hospital, Fasa University of Medical Sciences, Fasa, Iran
4- Noncommunicable Diseases Research Center, Fasa University of Medical Sciences, Fasa, Iran
5- Department of Pathology, School of Allied Medicine Sciences, Fasa University of Medical Sciences, Fasa, Iran
6- Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran
Abstract:   (681 Views)
CRISPR is an extraordinarily powerful technique regulating any target gene across the genome with promising therapy intentions. CRISPR-Cas9 is a convenient tool for gene manipulation. Notwithstanding this, the broad consequence of human gene editing, particularly germinal genes, cannot be predicted. Firstly, once edited, the genes would be part of the human population for successive generations and may be impossible to remove from humanity; secondly, success is not guaranteed; thirdly, the fidelity of editing, as it could affect unrelated genes or unspecified segments of DNA; and last but not least, its influence on gene interaction, network, and signaling pathways could be difficult to be predicted. CRISPR-Cas9 mostly includes precise genome editing, rapidity and cost-effectiveness, creation of disease models, study of gene function, applications in gene therapy and translation research and wide diversity for species. The technique also ignited the moral and ethical concerns of scientific community.  Ethics and safety approval for gene modification in human cells is required by the National Institutes of Health (NIH). The NIH does not currently fund studies of CRISPR in human embryos and opposes the CRISPR utilization in germline cells because these alterations would be permanent and heritable. The technology has promised with the most profound implications for cancer therapy. Recent advances in CRISPR-based technology is redefining how cancer is studied and potentially improves anti-cancer therapies. One way to improve the technology is to use machine-learning approaches to comprehending CRISPR errors and predicting more specific edits and repairing outcomes.
 
Keywords: CRISPR-Cas9, genome editing, animal modeling, advantages, disadvantages  
Full-Text [PDF 676 kb]   (180 Downloads)    
Type of Study: Review | Subject: Cellular-Molecular Biology
Received: 2023/02/9 | Accepted: 2023/09/9 | Published: 2024/01/28
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