Journal of Advanced Biomedical Sciences
JABS
Thu, Nov 21, 2024
[Archive]
Volume 14, Issue 3 (5-2024)
JABS 2024, 14(3): 232-243 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Raeeszadeh M, Afshari A, Kazemiafshar S, Kazemiafshar H, Amiri A A. Cytotoxicity of Ethanolic and Methanolic Extracts of Medicago sativa L. (Alfalfa) on K562 Myeloid Cancer Cell Lines. JABS 2024; 14 (3) :232-243
URL: http://jabs.fums.ac.ir/article-1-3095-en.html
URL: http://jabs.fums.ac.ir/article-1-3095-en.html
Mahdieh Raeeszadeh * 
1, Afsoon Afshari2 , Samaneh Kazemiafshar3 , Hajar Kazemiafshar3 , Ali Akbar Amiri4
1, Afsoon Afshari2 , Samaneh Kazemiafshar3 , Hajar Kazemiafshar3 , Ali Akbar Amiri4
1- Department of Basic Sciences, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran , vet_mr@yahoo.com
2- Department of Molecular Genetics, Shiraz Nephro-Urology Research Center, Shiraz University, Shiraz, Iran
3- Faculty of Veterinary Sciences, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran
4- Department of Basic Sciences, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran
2- Department of Molecular Genetics, Shiraz Nephro-Urology Research Center, Shiraz University, Shiraz, Iran
3- Faculty of Veterinary Sciences, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran
4- Department of Basic Sciences, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran
Abstract: (139 Views)
Background & Objectives: For centuries, the alfalfa (Medicago sativa L.) plant has been recognized for its versatile and active role in treating various diseases. Not only has it been utilized as a therapeutic agent, but it has also been served as a dietary component for both animals and humans. Given the distinctive attributes of this plant in ethnopharmacology, this study aimed to investigate the effects of ethanolic and methanolic extracts of M. sativa L. on the K562 myeloid cell line under in vitro conditions.
Materials & Methods: The phytochemical composition of M. sativa L. was determined through Gas Chromatography-Mass Spectrometry (GC-MS) analysis. Cell viability was assessed using the MTT assay, wherein K562 cells were subjected to varying concentrations (50–100 μg/mL) of methanolic and ethanolic extracts over 24, 48, and 72-hour intervals to determine the IC50. Subsequently, the most promising IC50 result was employed in flow cytometry (Flow Jo Software) analysis.
Results: Active constituents identified included phytol, phenol, linolenic acid, and glycine. Statistical analysis revealed a time-dependent but not dose-dependent effect. It was noteworthy that the IC50 for the methanolic extract after 72 hours was 9.45 µg/mL, whereas it was 19.3 µg/mL for the ethanolic extract. Flow cytometry analysis indicated that the methanolic extract caused 49.16% and the ethanolic extract caused 15.42% of cell death.
Conclusion: The results demonstrated that the ethanolic extract of alfalfa is more effective than the methanolic extract on the K562 cell line. Therefore, M. sativa L. potential application in myeloid cancer therapy can be investigated in more details.
Materials & Methods: The phytochemical composition of M. sativa L. was determined through Gas Chromatography-Mass Spectrometry (GC-MS) analysis. Cell viability was assessed using the MTT assay, wherein K562 cells were subjected to varying concentrations (50–100 μg/mL) of methanolic and ethanolic extracts over 24, 48, and 72-hour intervals to determine the IC50. Subsequently, the most promising IC50 result was employed in flow cytometry (Flow Jo Software) analysis.
Results: Active constituents identified included phytol, phenol, linolenic acid, and glycine. Statistical analysis revealed a time-dependent but not dose-dependent effect. It was noteworthy that the IC50 for the methanolic extract after 72 hours was 9.45 µg/mL, whereas it was 19.3 µg/mL for the ethanolic extract. Flow cytometry analysis indicated that the methanolic extract caused 49.16% and the ethanolic extract caused 15.42% of cell death.
Conclusion: The results demonstrated that the ethanolic extract of alfalfa is more effective than the methanolic extract on the K562 cell line. Therefore, M. sativa L. potential application in myeloid cancer therapy can be investigated in more details.
Type of Study: Research |
Subject:
Pharmacology
Received: 2024/08/7 | Accepted: 2024/09/10 | Published: 2024/11/2
Received: 2024/08/7 | Accepted: 2024/09/10 | Published: 2024/11/2
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
This work is licensed under a Creative Commons — Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)